Register – Size-Exclusion Chromatography: An Overview of the Principles and Techniques to Obtain Reliable Protein Size-Based Separations


Size-Exclusion Chromatography (SEC) is a powerful technique for protein characterization that requires an understanding of the principles to be successful.

In these Learning Modules, we will discuss the history and science of SEC, its strengths and limitations, and how to get the most from this important analytical technique. We’ll also discuss the analytical challenges with a focus on monoclonal antibody separations and the effective use of LC-based, size-exclusion chromatography to obtain reliable mAb aggregate, monomer, and fragment analyses.

The available tutorials in this course include:

  • [Part 1] Analytical Challenges and Use of SEC for mAbs
  • [Part 2] Principles and Practice of SEC for Reliable Monoclonal Antibody Analyses
  • [Part 3] Selecting Appropriate SEC Pore Size for Reliable Protein / Peptide Separations
  • [Part 4] Choosing Appropriate SEC Column Size based on LC System and Application Goals

The full course will comprise 6 parts, the final two of which will be available in the near future. Part 5 will be titled ‘Considerations to Maximize SEC Column Life’. If you register for the course we’ll send you updates when the new tutorials are ready to watch.

The Presenter
Bill Warren (Principal Product Manager, Waters)
Bill received his Master’s in Microbiology and Immunology from University of Louisville School of Medicine. In 1986, joined Waters Corporation as a senior applications chemist involved with the research and development of HPLC, FPLC, and Capillary Electrophoresis applications for the analysis and purification of proteins, peptides, and nucleic acids. Bill currently is a Principal Bioseparation Columns Product Manager whose responsibilities involve assisting in the development and commercialization of innovative reagents and chromatography-based technologies for the separation, purification, and characterization of biological molecules